Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | in 10% (v/v) ethanol, the enzyme significantly increases its activity by 50% | Homarus americanus | |
Isopropanol | the presence of 15% (v/v) isopropanol generates a significant increase of 80% on enzyme activity | Homarus americanus | |
methanol | exposure of the enzyme to 5-15% (v/v) methanol produces 50% increase in enzyme activity | Homarus americanus |
General Stability | Organism |
---|---|
the non-ionic detergents Tween-20 and Triton X-100 stabilize the enzyme activity. The enzyme is active until 2.5 M urea and is resistant to proteolysis by papain and renin. Glycerol (2.5-30% (v/v)) shows no stabilization effect on the activity of the enzyme. In 25% (v/v) methanol, the enzyme retains 100% of the activity | Homarus americanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | in the presence of 10% (v/v) DMSO a significant decrease in activity to 40% is observed. In 25% (v/v) DMSO the enzyme maintains 20% of the activity. Activity is completely lost when the enzyme is exposed to DMSO at concentration above 35% (v/v) | Homarus americanus | |
ethanol | at 25 and 35% (v/v) ethanol, enzyme activity decreases to 60 and 40%, respectively. In 50% ethanol, the enzyme completely loses its activity | Homarus americanus | |
Isopropanol | at 25% (v/v) isopropanol, the enzyme activity drops to 50%, enzyme activity is completely lost at 35% (v/v) | Homarus americanus | |
methanol | the enzyme loses activity in the presence of more than 35% (v/v) methanol | Homarus americanus | |
NaCl | purified enzyme retains more than 50% of proteolytic activity in final concentration of 1 M NaCl and 20% in 1.5 M NaCl | Homarus americanus | |
Triton X-100 | 60% residual activity at 0.001-0.01% (v/v) Triton X-100, below 20% residual activity at 0.0005% or 0.05-0.1% (v/v) Triton X-100 | Homarus americanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homarus americanus | - |
American lobster | - |
Purification (Comment) | Organism |
---|---|
pepstatin A-agarose column chromatography, NAP-5 Sephadex G25 gel filtration, and UNO Q1 column chromatography | Homarus americanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric juice | - |
Homarus americanus | - |
Storage Stability | Organism |
---|---|
25°C, purified and concentrated enzyme (0.005 mg/ml) at pH 4.5, 7 days, no loss of activity | Homarus americanus |
25°C, purified and low-concentrated enzyme (0.00005 mg/ml) at pH 4.5, 8 h, complete loss of activity | Homarus americanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
((7-methoxycoumarin-4-yl)acetyl)-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys-(2,4-dinitrophenyl)-D-Arg-amide + H2O | - |
Homarus americanus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Homarus americanus |
Synonyms | Comment | Organism |
---|---|---|
cathepsin D1 | - |
Homarus americanus |
CD1 | - |
Homarus americanus |